Total bile acids 21 FS – New DiaSys publication

The number of people affected by chronic inflammatory bowel disease (IBD) is continuously increasing, with lifestyle in industrialized countries (e.g. hygiene and diet) being suspected as the cause.

The determination of bile acids (BA) in stool samples is therefore becoming increasingly important as a screening parameter and for monitoring the course of IBD. However, the current method is time-consuming and resource-intensive, costly and requires a high level of patient compliance. Alternatively, the clinical-chemical determination of bile acids in stool samples is possible.

The 5th generation cycling method has already been thoroughly validated as an assay for the determination of GS in serum samples. The extension of this method to stool samples seems to be a logical further development with many advantages. Unfortunately, this approach is not yet sufficiently utilized.

In a recent publication in “Clinical Chemistry and Laboratory Medicine” an evaluation was performed comparing the DiaSys Total bile acids 21 FS assay with competing assays using HPLC-MS/MS as a reference for individual GS. The results showed that the DiaSys assay had a more accurate recovery of individual bile acids, despite the strong correlation between the two assays for total bile acids. This result indicates that Total bile acids 21 FS has a lower dispersion of the recovery of individual bile acids. This property is crucial for the accurate diagnosis of pathological samples.

For a more detailed insight, please click the following link to read the publication:
Towards routine high-throughput analysis of fecal bile acids: validation of an enzymatic cycling method for the quantification of total bile acids in human stool samples on fully automated clinical chemistry analyzers

For further Information on Total bile acids 21 FS in conjunction with IBD please use the following links:

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Total bile acids 21 FS