Glucose-6-Phosphate Dehydrogenase

Individuals with G6PDH deficiency are at risk of hemolytic anemia in states of oxidative stress. Oxidative stress can result from infection, chemical exposure to medication or certain food. Broad beans, e.g., fava beans, contain high levels of vicine, divicine, convicine and isouramil, all of which are oxidants. In newborn, G6PDH deficiency may be responsible of neonatal jaundice.

Main assay features

• Method: Ultraviolet, kinetic (quantitative)

• Samples: Whole blood collected with EDTA, heparin or ACD (Acid-Citrate-Dextrose)

• Linearity: 3200 U/L

• Sensitivity (LoD): 27 U/L

• Precision (run-to-run): <2.0%

• Accuracy : Excellent, fulfills even the more demanding Ricos criteria

Method Comparison - DiaSys vs Competitor
Evaluation of bias - DiaSys vs Competitor

The format of the reagent kits is adapted to both manual and automated techniques, adaptations on different analyzers are available on request.

Product

Cat. No.

Kit

Reagent
R1: BUFFER: 1 x 20 mL 
R2: NADP: 4 x 5 mL
R3: G6P (ready to use): 1 x 40 mL

1 7900 99 10 026

4 x 15 mL

G6PDH Red Cell Lysing Reagent
(only for automation)

1 7900 99 10 113

4 x 25 mL

TruCal G6PDH 2 levels

1 7900 99 10 040

2 x 0.5 mL

TruLab G6PDH 3 levels

1 7900 99 10 045

3 x 0.5 mL

Main advantages

• Preparation of red cell hemolysate without blood treatment with 0.9% NaCl solution

• Lysing reagent is triton free

• Wide linearity range

• Excellent precision and accuracy

¹Mégarbane. Déficit en glucose-6-phosphate déshydrogénase: quand y penser et quelles précautions prendre? Glucose-6-phosphate dehydrogenase deficiency; Réanimation (2008) 17, 399-406.